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24-well quantification of (A) Mean firing rate (Hz), (B) Number of active electrodes, (C) Network burst frequency (Hz), (D) Network burst duration (s), (E) Network interburst interval coefficient of variation, and (F) Synchrony index on DIV 11, 17, 26, and 33. (G) Representative raster plots. Statistical analysis was performed with repeated measures ANOVA and Bonferroni corrections Error bar: SEM. N=24 independent wells. p-value < 0.05 *, p-value < 0.01 **, p-value < 0.001 ***

Journal: bioRxiv

Article Title: Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes

doi: 10.1101/2024.03.04.583341

Figure Lengend Snippet: 24-well quantification of (A) Mean firing rate (Hz), (B) Number of active electrodes, (C) Network burst frequency (Hz), (D) Network burst duration (s), (E) Network interburst interval coefficient of variation, and (F) Synchrony index on DIV 11, 17, 26, and 33. (G) Representative raster plots. Statistical analysis was performed with repeated measures ANOVA and Bonferroni corrections Error bar: SEM. N=24 independent wells. p-value < 0.05 *, p-value < 0.01 **, p-value < 0.001 ***

Article Snippet: Multielectrode array 24-well plates containing 16 electrodes per well were obtained from Axion Biosystems (CytoView MEA plate).

Techniques:

Characterized by (A) Mean firing rate (Hz) (B) Number of active electrodes (C) Network burst frequency (Hz) (D) Average network burst duration (s) (E) Network interburst interval coefficient of variation (F) Synchrony index. Statistical analysis was performed with an independent t-test. Error bar: SEM. N=24 independent wells per plate. p-value < 0.05 *, p-value < 0.01 **, p-value < 0.001 ***

Journal: bioRxiv

Article Title: Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes

doi: 10.1101/2024.03.04.583341

Figure Lengend Snippet: Characterized by (A) Mean firing rate (Hz) (B) Number of active electrodes (C) Network burst frequency (Hz) (D) Average network burst duration (s) (E) Network interburst interval coefficient of variation (F) Synchrony index. Statistical analysis was performed with an independent t-test. Error bar: SEM. N=24 independent wells per plate. p-value < 0.05 *, p-value < 0.01 **, p-value < 0.001 ***

Article Snippet: Multielectrode array 24-well plates containing 16 electrodes per well were obtained from Axion Biosystems (CytoView MEA plate).

Techniques:

(A) Representative raster plots and histograms of relative population firing rates for baseline, 24 hours after treatment, and after 2 washouts. (B) Mean firing rate (Hz) (C) Number of active electrodes (D) Network burst frequency (Hz) (E) Network burst percentage (%) (F) Synchrony Index (G) Network Interburst Interval Coefficient of Variation. Statistical analysis was performed with repeated measures ANOVA and Bonferroni corrections. Error bar: SEM. N=4 independent wells. p-value < 0.05 *, p-value < 0.01 **, p-value < 0.001 ***

Journal: bioRxiv

Article Title: Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes

doi: 10.1101/2024.03.04.583341

Figure Lengend Snippet: (A) Representative raster plots and histograms of relative population firing rates for baseline, 24 hours after treatment, and after 2 washouts. (B) Mean firing rate (Hz) (C) Number of active electrodes (D) Network burst frequency (Hz) (E) Network burst percentage (%) (F) Synchrony Index (G) Network Interburst Interval Coefficient of Variation. Statistical analysis was performed with repeated measures ANOVA and Bonferroni corrections. Error bar: SEM. N=4 independent wells. p-value < 0.05 *, p-value < 0.01 **, p-value < 0.001 ***

Article Snippet: Multielectrode array 24-well plates containing 16 electrodes per well were obtained from Axion Biosystems (CytoView MEA plate).

Techniques: